Where a TIFF keeps its pixel size
The viewer reports which of these it found. If none is present, set the scale yourself (the pixel size calculator explains how) before measuring.
Fields that can identify a patient or a lab
Whole-slide files often carry more than pixels: a photo of the glass label (barcode, case number, sometimes a name), a macro image of the whole slide, scan date and time, the scanner's serial number, the user name and the original file name. The tool lists those it finds in the main tags. Before sharing a slide outside your institution, remove them: see how to remove labels and patient data from slide images.
The same information from the command line
tiffinfo file.tif (libtiff) prints every directory; exiftool -a -G1 file.tif adds maker tags; in Python, tifffile.TiffFile(path) exposes .pages[i].tags, .ome_metadata and .imagej_metadata. For SVS, openslide.OpenSlide(path).properties gives the parsed fields (see reading SVS files in Python).
How it opens a file without uploading it
When you choose a file, the browser hands this page a handle to it on your disk. The page reads only the bytes it needs: the TIFF directory first, then the tiles for the part of the image on screen, and decodes them in the page with an open-source TIFF reader (geotiff). Nothing is sent to a server, so unpublished data and slides with patient labels stay on your computer, and multi-gigabyte slides open in seconds because they are never copied anywhere. Close the tab and the file is gone from the page.
The page loads its own scripts from slidescope.science and the site's privacy-respecting page-view counter; neither ever sees your image. Open-source licences: image-tools-vendor.LICENSE.txt.
Questions
How do I see the metadata of a TIFF file?
Open it in this page to list every tag of every image, or use tiffinfo, exiftool or tifffile in Python. The ImageDescription tag holds most microscopy metadata (OME-XML, ImageJ or Aperio fields).
How do I find the pixel size of a TIFF?
Look for PhysicalSizeX in the OME-XML, MPP in an Aperio description, or the ImageJ unit with XResolution. A plain 72 or 300 dpi resolution is a print setting, not a microscope calibration.
Can I export the tags?
Yes: Download all tags as JSON saves the tags of up to 200 images in the file, with long arrays shortened.
Is my file uploaded?
No. The browser reads the file on your computer; nothing is sent anywhere.
Is this tool free?
Yes, it is free and needs no account. SlideScope, the desktop microscopy app made by the same team, is a paid app with a free sample library (every tool) and a 3-day free trial for your own images.
Need more than a browser can do?
SlideScope opens 34 microscope and slide-scanner formats on Windows and Mac, CZI, ND2, LIF, MRXS and JPEG 2000 SVS among them, with measuring, annotations, cell counting and full-resolution OME-TIFF export. Try every tool free on the built-in samples, then open your own images with the 3-day free trial. Already decided? Subscribe now.
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