microscopy image viewer

Supported microscopy and whole-slide file formats

Start with the files you use. Check support and important limitations before bringing them into your workflow.

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CZI · ND2 · SVS · DICOM · TIFFWindows · macOSSlideScope 1.10.1

Supported file formats

CZI for Zeiss microscopy images
ND2 for Nikon time-lapse, Z-stack, and multidimensional microscopy files
SVS for Aperio whole-slide pathology images
NDPI, VMS, VMU, MRXS, SCN, BIF, SVSLIDE, QPTIFF, and AVS scanner slides from Hamamatsu, 3DHISTECH, Leica, Ventana, Aperio, Vectra, and Agilent ARGOS
DICOM for research and education medical imaging review
TIFF and microscopy TIFF export workflows
LIF for Leica confocal and widefield microscopy files
IMS for Imaris/Bitplane multi-channel imaging files
Olympus/Evident VSI, OIB/OIF and OIR, Zeiss ZVI and LSM, Leica LOF and XLEF, DeltaVision DV, MetaMorph STK and ND, Hamamatsu NDPIS and OME-Zarr (including HCS plates), with the instrument settings each file records
PNG, JPEG, JPEG 2000, BMP, GIF and WebP images

Medical imaging

pathology slide viewer
annotations
calibrated measurements
AI Analysis

Fast, human support

Have a file that won't open, or a format we don't list yet?

Send us the details. Microscopy files come from many instruments, scanners, and software versions, so we actively investigate compatibility issues, and our support team is available 24/7. One unusual file rarely means SlideScope cannot fit your workflow, and helpful reports may qualify for subscription rewards. If the format you need is not on our list, tell us which instrument or software wrote it.

Report a file or format

FAQ

What is SlideScope?

SlideScope is a desktop microscopy and medical imaging viewer for Windows and macOS. It supports CZI, ND2, SVS, DICOM, and TIFF review workflows.

Who is it for?

It is built for research labs, microscopy core facilities, educators, students, and pathology review teams that need practical file review, annotations, measurements, metadata, and export.

What if a file doesn't open or render correctly?

Tell us. Send the file type, the microscope or software that produced it, and what you saw, and a real person from our 24/7 support team will reply. Unusual files, incorrect rendering, and workflows that don't behave as expected are exactly the reports that help us improve SlideScope, and helpful reports can earn rewards toward your subscription.