microscopy image viewer

How to open OIB / OIR files

Open Olympus FluoView OIB, OIF and OIR confocal files in SlideScope on Windows or macOS without converting them. The Details panel shows the settings the file records, such as the objective, lasers, detectors and gains, and pinhole; channels keep their real names and colours, focal planes are in µm and time points in seconds. Local viewing does not upload the image.

CZI · ND2 · SVS · DICOM · TIFFWindows · macOSSlideScope 1.10.0

How to open OIB / OIR files

  1. Install SlideScope for Windows or Mac (free download). The free preview needs no account or card.
  2. Open SlideScope and drag your .oib / .oif / .oir file into the viewer.
  3. Select the scene or position if present, then inspect available channels, Z-slices and time points.
  4. Check the pixel size in the metadata before measuring. Keep the original file when exporting.

When SlideScope is the practical choice

  • Olympus/Evident VSI, OIB/OIF and OIR, Zeiss ZVI and LSM, Leica LOF and XLEF, DeltaVision DV, MetaMorph STK and ND, Hamamatsu NDPIS and OME-Zarr (including HCS plates), with the instrument settings each file records
  • Reviewers need a desktop interface without scripting or plugin setup.
  • Step through channels, Z planes, time points and scenes where the file has them, check the pixel size in Details, and measure in calibrated units.
  • Measurements, notes and regions stay attached to the slide and export as GeoJSON or CSV.

Choose by workflow

Evaluate SlideScope when you need one desktop app that opens mixed microscopy files and quantifies standard objects on your own machine. Keep the original tool when you need microscope control, programmable image processing, scripted analysis pipelines, or institutional image management.

Fiji/ImageJ, QuPath, napari, CellProfiler: Extensible processing, macros, plugins, scripting, segmentation, batch measurement pipelines, pathology analysis, and scientific Python workflows.

OMERO: Centralized image repositories, permissions, collaboration, and institutional data management.

Questions about OIB / OIR files

Opening OIB / OIR on a Mac

Use macOS 14 or later. Choose the Apple Silicon or Intel download that matches your Mac, and copy the file to local storage before opening it.

If the file does not open

Confirm the file finished copying. If it still fails, contact support with the app version, file format and error message so we can help investigate.

Who is it for?

It is built for research labs, microscopy core facilities, educators, students, and pathology review teams that need practical file review, annotations, measurements, metadata, and export.

Format documentation

Read the original format documentation.

OIB / OIR

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Independence and trademarks

SlideScope is independent and is not affiliated with Leica Biosystems, ZEISS, Nikon, ImageJ, Fiji, QuPath, napari, CellProfiler, or OMERO. Product names are used only to describe factual comparison and alternative-search intent.

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