microscopy image viewer

How to open NDPI files

You can open Hamamatsu NDPI files with NDP.view2, Hamamatsu’s free viewer for Windows and macOS; with QuPath, which is free on Windows, Mac and Linux; or with SlideScope, a desktop viewer for Windows and Mac with a free sample library. The steps below use SlideScope.

CZI · ND2 · SVS · DICOM · TIFFWindows · macOSSlideScope 1.23.0

How to open NDPI files

  1. Install SlideScope for Windows or Mac (free download). The built-in sample library is free with every tool; opening your own slides needs a subscription.
  2. Drag the .ndpi file into the viewer or choose File › Open File… Open scanner slides from local storage.
  3. Use the overview to jump to a region, then zoom in. SlideScope loads the pyramid level that matches what is on screen.
  4. Check the pixel size (µm/px) in the file details before measuring, then measure, annotate and export the current view as an image.

Ways to open NDPI files

ToolCostPlatformsBest for
Hamamatsu NDP.view2Free with Hamamatsu slides (NDP.view2 Plus is paid)Windows 10/11, macOS 13–26 (Apple silicon and Intel)The vendor’s own viewer for NDPI
QuPathFree, open sourceWindows, macOS, LinuxWhole-slide analysis projects and scripting
SlideScopeFree samples with every tool, then a subscription from $35/month for your own imagesWindows 10+, macOS 14+ (Apple silicon and Intel)Viewing, measuring and annotating NDPI alongside CZI, ND2, SVS, NDPI, MRXS, DICOM and TIFF, with files kept on your computer

Open Hamamatsu NDPI whole-slide images in SlideScope on Windows or macOS without converting them. Pan and zoom across the full slide, measure in µm using the scanner’s calibration, and add annotations. Local viewing does not upload the image.

Source: Whole slide image viewer comparison (platforms and prices, each linked to its source).

See also: Hamamatsu NDP.view2: where to download it, Mac support, and the alternatives.

When SlideScope is the practical choice

  • NDPI, VMS, VMU, MRXS, SCN, BIF, SVSLIDE, QPTIFF, and AVS scanner slides from Hamamatsu, 3DHISTECH, Leica, Ventana, Aperio, Vectra, and Agilent ARGOS
  • Reviewers need a desktop interface without scripting or plugin setup.
  • Step through channels, Z planes, time points and scenes where the file has them, check the pixel size in Details, and measure in calibrated units.
  • Measurements, notes and regions stay attached to the slide and export as GeoJSON or CSV.

Choose by workflow

Evaluate SlideScope when you need one desktop app that opens mixed microscopy files and quantifies standard objects on your own machine. Keep the original tool when you need microscope control, programmable image processing, scripted analysis pipelines, or institutional image management.

Fiji/ImageJ, QuPath, napari, CellProfiler: Extensible processing, macros, plugins, scripting, segmentation, batch measurement pipelines, pathology analysis, and scientific Python workflows.

OMERO: Centralized image repositories, permissions, collaboration, and institutional data management.

Questions about NDPI files

Is there a free way to open NDPI files?

Yes. Hamamatsu’s NDP.view2 and QuPath are free. SlideScope is free to download, the built-in sample library is free with every tool, and the app checks for free whether your own files open; opening your own images needs a subscription, from $35/month.

Opening NDPI slides on a Mac

SlideScope runs natively on macOS 14 or later, so you do not need a Windows PC or virtual machine for the vendor viewer. Choose the Apple Silicon or Intel download and copy the slide to local storage before opening it.

If the slide does not open

Confirm the whole file finished copying to local storage. If it still fails, contact support with the app version, file format and error message so we can investigate.

Who is it for?

It is built for research labs, microscopy core facilities, educators, students, and pathology review teams that need practical file review, annotations, measurements, metadata, and export.

Format documentation

Read the original format documentation.

NDPI

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Independence and trademarks

SlideScope is independent and is not affiliated with Leica Biosystems, ZEISS, Nikon, ImageJ, Fiji, QuPath, napari, CellProfiler, or OMERO. Product names are used only to describe factual comparison and alternative-search intent.

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